Fig. 2: PROTEUS generates authentic evolution products.

Titers (a) and (b) amplification factors of VLVs propagated on cells expressing VSVG under the control of TRE3G (gray box) (N = 4 biological replicates). RP (packaging) and RA (amplification) indicate rounds of VLV propagation under constitutive VSVG expression, while E1-X labeling indicates rounds of evolution under transgene-regulated VSVG expression. Red bars indicate the RT-qPCR NTC signal in (a). Statistical comparisons were made using two-tailed unpaired t-tests. c Allele frequency of the major variants identified in Campaign 1. d Dox-resistance of evolved tTA variants (N = 5 biological replicates). e Variant-induced structural changes in tTA modeled with AlphaFold2 (red, mutated residues; blue, displaced functional groups; dashed green circle, drug binding pocket). Source data are provided as a Source Data file.