Fig. 2: 3C-mRNA inhibits the proliferation, migration and invasion of GBM cells and promotes cell apoptosis.
From: Gene Therapy with Enterovirus 3 C Protease: A Promising Strategy for Various Solid Tumors
U87 MG and U138 MG cells were transfected with 3C-mRNA (0.5 μg/mL, 1 μg/mL, or 1.5 μg/mL), 3 C(C147S)-mRNA (1.5 μg/mL), or 3C-mRNA (1.5 μg/mL) + AG7088 (10 μM). a Cell migration ability of U87 MG and U138 MG cells subjected to different treatments, as determined by an in vitro Transwell migration assay. b Cell invasion ability of U87 MG and U138 MG cells subjected to different treatments, as determined by an in vitro Transwell invasion assay. Scale bar: 338.89 μm. c, d Apoptosis was assessed 24 h after transfection with different concentrations of 3C-mRNA, 3 C(C147S)-mRNA, or 3C-mRNA+AG7088. The data are presented as the means ± SDs. n = 3 independent biological samples. Statistical differences were assessed using one-way ANOVA with the Dunnett multiple comparisons test. **P < 0.01, ***P < 0.001, ****P < 0.0001, n.s. = not significant. Source data are provided as a Source Data file.
