Fig. 8: Schematic illustration of apoptosis and tumor regression induced by EV 3C-mRNA.
From: Gene Therapy with Enterovirus 3 C Protease: A Promising Strategy for Various Solid Tumors
The enterovirus 3 C protease mRNA was encapsulated into lipid nanoparticles to produce the 3C-LNPs, which efficiently induce tumor cell apoptosis by directly degrading hnRNP A1 and then activating caspase-3. Three dosing routes were tested in different mouse tumor models, each of which was shown to be highly efficacious in vivo. Intracranial (i.c.), subcutaneous (s.c.), or intravenous (i.v.) delivery routes were used for mouse tumor models of glioblastoma in the brain, breast cancer in the mammary gland, or hepatoma in the liver, respectively.
