Fig. 4: Optogenetic activation of PV or SST interneurons suppresses social interactions in the three-chamber. | Nature Communications

Fig. 4: Optogenetic activation of PV or SST interneurons suppresses social interactions in the three-chamber.

From: Anterior cingulate cortex parvalbumin and somatostatin interneurons shape social behavior in male mice

Fig. 4: Optogenetic activation of PV or SST interneurons suppresses social interactions in the three-chamber.The alternative text for this image may have been generated using AI.

a Traces of PV-Cre mice in the sociability test. b, c Social interaction time (b) and discrimination score (c) from the sociability test (n = 9 mice, Wilcoxon signed-rank test, Z = −2.666, P = 0.008 for OFF1; two-tailed paired t test, t = −2.516, P = 0.036 for ON; two-tailed paired t test, t = 1.544, P = 0.161 for OFF2; two-tailed paired t test, t = 6.805, P = 0.0001 for discrimination score). d Traces of PV-Cre mice in the social preference test. e, f Same as (b, c) but in the social preference test (n = 9 mice, two-tailed paired t test, t = 6.831, P = 0.0001 for OFF1; t = 0.827, P = 0.432 for ON; t = 0.830, P = 0.431 for OFF2; t = 3.833, P = 0.005 for discrimination score). g Same as (a) but for SST-Cre mice. h, i Same as (b, c) but for SST-Cre mice (n = 10 mice, two-tailed paired t test, t = 4.804, P = 0.001 for OFF1; t = 0.236, P = 0.819 for ON; t = 4.666, P = 0.001 for OFF2; t = 3.686, P = 0.005 for discrimination score). j Same as (d) but for SST-Cre mice. k, l Same as (e, f) but for SST-Cre mice (n = 10 mice, Wilcoxon signed-rank test, Z = −2.803, P = 0.005 for OFF1, Z = −2.191, P = 0.028 for ON; two-tailed paired t test, t = −1.110, P = 0.296 for OFF2, t = 6.661, P < 0.0001 for discrimination score). m Delay time when the first social behavior stopped during the sociability test (n = 9 PV-Cre mice, n = 8 SST-Cre mice; two-tailed unpaired t test, t = −2.816; P = 0.013). n Comparison of normalized discrimination scores in the sociability test (n = 9 PV-Cre mice, n = 10 SST-Cre mice, two-tailed unpaired t test, t = −1.863, P = 0.079). o Delay time when the first social behavior stopped in the stranger zone (n = 9 PV-Cre mice, n = 10 SST-Cre mice, Mann‒Whitney U test, Z = −2.195, P = 0.028). p, q Time spent in the center zone for PV-Cre (p) and SST-Cre (q) in the social preference test (n = 9 mice, repeated-measures ANOVA, P = 0.001 for PV; n = 10 mice, Friedman’s M test, Χ2 = 3.800, P = 0.150 for SST). r Same as (n) but in the social preference test (n = 9 PV-Cre mice, n = 10 SST-Cre mice; two-tailed unpaired t test, t = −3.164, P = 0.006). All statistical tests are two-sided. Data are presented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Source data are provided as a Source Data File.

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