Fig. 1: Gemcitabine induced the expression of SERPINB9 in pancreatic cancer. | Nature Communications

Fig. 1: Gemcitabine induced the expression of SERPINB9 in pancreatic cancer.

From: Rational development of gemcitabine-based nanoplatform for targeting SERPINB9/Granzyme B axis to overcome chemo-immune-resistance

Fig. 1

a Volcano plot for the RNA-seq analysis of KPC-C2 gemcitabine resistant (GEMR) cells versus KPC-C2 wide type (WT) cells. Statistical analysis of RNA-seq was performed by two-tailed Student’s t-test for comparison using Cuffdiff in the Cufflinks package. b qRT-PCR analysis of the fold changes of 8 genes selected from RNA-seq (a) in KPC-C2 GEMR, KPC-C5 GEMR, and Panc02 GEMR versus their WT cells. c qRT-PCR analysis of Serpinb9 expression in KPC-C2 GEMR, KPC-C5 GEMR, and Panc02 GEMR versus their WT cells. n = 6 independent experiments. d SERPINB9 expression in pancreatic adenocarcinoma (PAAD) patients’ tumor tissues (n = 179) and normal tissues (n = 171) from TCGA database. Center lines represent median expressions, the box limit indicates the lower quantile and upper quantile, and whiskers represent the minimal and maximal expressions. e Survival rate of PAAD patients with high levels of SERPINB9 versus those with low levels of SERPINB9 from TCGA database. Serpinb9 mRNA expression in KPC-C2 (f), KPC-C5 (g), or Panc02 (h) cells following treatment with various concentrations of gemcitabine. n = 3 independent samples. i Serpinb9 protein expression in KPC-C2, KPC-C5 or Panc02 cells following treatment with various concentrations of gemcitabine. j SERPINB9 mRNA expression in PANC-1, MIA PaCa-2, Panc 10.05, and Panc 02.03 cells following treatment with different concentrations of gemcitabine. n = 3 independent samples. Representative Western blot of SERPINB9 levels (k) in PANC-1, MIA PaCa-2, Panc 10.05 and Panc 02.03 cells after treatment with different concentrations of gemcitabine, and densitometry analysis of the protein bands in the Western blot (l). n = 3 independent samples and data were quantified by densitometry. Data are presented as mean ± s.e.m. in (c, f, g, h, j). Statistical analysis was performed by two-tailed Student’s t-test for comparison in (c), log rank test for comparison in (e) and one-way ANOVA with Tukey’s post hoc test for comparison in (f, g, h, j, l). Source data are provided as a Source Data file.

Back to article page