Fig. 7: Naive and primed hESCs exhibit both similarities and differences in their response to CBA conditions. | Nature Communications

Fig. 7: Naive and primed hESCs exhibit both similarities and differences in their response to CBA conditions.

From: Deciphering signaling mechanisms and developmental dynamics in extraembryonic mesoderm specification from hESCs

Fig. 7: Naive and primed hESCs exhibit both similarities and differences in their response to CBA conditions.The alternative text for this image may have been generated using AI.

a Schematic of CBA treatment of naive hESCs. b Brightfield image illustrating CBA-treated naive hESCs on day 5 (CBAN-D5). c IF staining images for the indicated markers in CBAN-D5. d Heatmap illustrating the expression of representative pluripotency, PS, ExM, and AM marker genes across the differentiation time courses of CBANs. e UMAP visualization of single-cell transcriptomics from CBA-treated AIC-N4 hESCs on day 5 (CBAN4-D5). UMAP plot is color-coded according to cell cluster identity annotations. HEP, haemato-endothelial progenitor. f UMAP plots of the indicated genes expressed in CBAN4-D5. g IF staining images for the hematopoietic markers KDR, CD31, and CD34 in CBAN-D5 and CBAP-D5, naive and primed hESCs were cultured under the indicated conditions. h Schematic of FACS for MESP1-mTomato+ PSLIs. i Flow cytometry analysis of MESP1-mTomato expression across the differentiation time courses of CBANs. j Brightfield and fluorescence images of sorted PSLIs at 72 hours. k IF staining of the indicated markers in sorted PSLIs after 48 hours of additional culture under CBA conditions. l Schematics illustrating the signaling principles and developmental dynamics in ExM specification from naive and primed hESCs. Scale bars, 100 µm. Source data are provided as a Source Data file. See also Supplementary Fig. 7.

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