Fig. 3: CYP26 inhibition phenocopies exogenous RA during limb regeneration. | Nature Communications

Fig. 3: CYP26 inhibition phenocopies exogenous RA during limb regeneration.

From: Retinoic acid breakdown is required for proximodistal positional identity during axolotl limb regeneration

Fig. 3: CYP26 inhibition phenocopies exogenous RA during limb regeneration.The alt text for this image may have been generated using AI.

A Timeline of TAL experiments and tissue collection timepoints. B Brightfield images of regenerates and skeletal structures of PBs and DBs treated with DMSO or 0.1, 1, or 5 µM TAL. Dashed lines indicate amputation plane. Scale bar = 2 mm. C 10 DPA DBs from RA reporter animals treated with DMSO or 1 µM TAL (3 cm (HT) animals aged 2 months). Dashed lines indicate amputation plane. Scale bar = 500 µm. D qRT-PCR of Gfp in tissue from RA reporter animals. (3.5 cm (HT) animals aged 2.5 months, 10 DPA). Boxplot and analyses as in Fig. 1B, C. *** = p < 0.001. E HCR-FISH for Cyp26a1 and Cyp26b1 in DBs administered 1 µM TAL at 14 DPA (3.5 cm (HT) animals aged 2.5 months). Dashed line indicates amputation plane. AF = autofluorescence. Scale bar = 200 µm or 20 µm (inset). F Dot quantification for mesenchymal and epithelial Cyp26a1 and Cyp26b1 in DBs treated with DMSO or 1 µM TAL at 14 DPA (3.5 cm (HT) animals aged 2.5 months). Axes and analyses as in Fig. 1E. * = p < 0.05. G 14 DPA DBs from Hoxa13:mCHERRY reporter animals treated with DMSO or 1 µM TAL (7.5 cm (HT) animals aged 6 months). Dashed lines indicate amputation plane. Scale bar = 500 µm. Sample size and exact p-values are located within the source data file. Source data are provided as a source data file.

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