Fig. 3: MTBP and pRecQL4 association with dormant origins.
From: Selective interactions at pre-replication complexes categorize baseline and dormant origins
A Effect of the MCM2S108A mutation on interactions with MTBP and pRecQL4. Experimental procedure: HCT116 cells with MCM2-mAID were complemented with WT or S108A MCM2 constructs. IAA treatment (500 μM for 16 h) depleted endogenous MCM2-mAID, enabling the incorporation of WT or S108A MCM2 into the pre-replication complex. Treatment with the SIRT1 inhibitor Ex527 (1 mM every 24 h for 2 days) activates dormant origins in cells complemented with the intact, but not the S108A mutated MCM215. B Replication origin binding of MTBP (left) and pRecQL4 (right) in unperturbed and Ex527 treated (1 mM every 24 h for 2 days) HCT116 cells harboring either WT-MCM2 or S108A-MCM2. Baseline and dormant origins were categorized as described in the legend to Fig. 1A, B.
