Fig. 4: Spp1 + Macrophages cause TAS CD8 + T cell dysfunction.
From: SPP1 + macrophages cause exhaustion of tumor-specific T cells in liver metastases
A Frequency of Spp1 + macrophages in intrahepatic (n = 8 mice) and subcutaneous TILs (n = 5 mice); p = 0.0016. B PDL1 + frequency of Spp1 + macrophages in intrahepatic (n = 8 mice) and subcutaneous TILs (n = 5 mice); p = 0.0428. C CD163 + CD206 + (M2) frequency of Spp1 + macrophages in intrahepatic (n = 8 mice) and subcutaneous TILs (n = 5 mice); p = 0.0019. D Correlation between orthotopic tumors and frequency of Spp1 + macrophages (n = 13). E Frequency of Spp1 + macrophages after hypoxia and normoxia (n = 4 mice); p =0.0186. F FN1 + frequency of macrophages after hypoxia and normoxia (n = 4 mice); p = 0.0466. G CD163 + CD206 + (M2) frequency of macrophages after hypoxia and normoxia (n = 4 mice); p = 0.0173. H Spp1 + macrophage expression of FN1 (MFI) after hypoxia and normoxia (n = 4 mice). Nanostring (I) T cell exhaustion, (J) JAKSTAT pathway, (K) MAPK, (L) NF-kB, and (M) TLR scores in IgG, Spp1, and Spp1 with anti-CD44 antibody (n = 6; box plots showing both replicates as bounds of box and center indicating mean). Experiments were completed in female, BALB/c 6–8 week old mice (A–H) and female, C57BL/6 6–8 week old mice (I–M). Data was analyzed using ordinary one way Anova (A–C), two-sided paired t test (E–H), simple linear regression (D): *P < 0.05; **P < 0.01; ***P < 0.005; ****P <0.001, ns = not significant (data are presented as mean values +/− SEM).
