Fig. 5: Knock out Spp1 decreases TAS CD8 + T cell exhaustion and tumor burden.
From: SPP1 + macrophages cause exhaustion of tumor-specific T cells in liver metastases
Symbol legend for (A–D) can be found in (D). A Frequency of CD39 + TRP2 + (TAS) CD8 + T cells (left; p = 0.0165) and CD39 + TRP2- (TAS) CD8 + T cells (right; p = 0.4001) in Spp1 KO compared to WT mice. B Frequency of TIM-3+ TRP2 + CD8 + T cells (left; p = 0.0464) and TIM-3 + TRP2- CD8 + T cells (right; p = 0.9834) in Spp1 KO (n = 6) compared to WT mice (n = 7). C Frequency of CD44 + CD62L- TRP2 + CD8 + T cells (left; p = 0.0338) and CD44 + CD62L- TRP2- CD8 + T cells (right; p = 0.3264) in Spp1 KO (n = 6) compared to WT mice (n = 7). D Frequency of CD44- CD62L + TRP2 + CD8 + T cells (left; p = 0.0229) and CD44- CD62L + TRP2- CD8 + T cells (right; p = 0.0986) in Spp1 knockout (KO) (n = 6) compared to WT mice (n = 7). E RIL-175 tumor weight comparing in Spp1 KO (n = 5) compared to WT mice (n = 5; p = 0.0428). Experiments (A–E) were completed in male and female B6.129S6(Cg)-Spp1tm1Blh/J 6–8 week old mice. Data was analyzed using 2-way Anova (A–D) and two-sided unpaired t test (E): *P < 0.05; **P < 0.01; ***P < 0.005; ****P < 0.001, ns = not significant (data are presented as mean values +/− SEM).
