Fig. 7: Exhausted, profibrotic polarization of the liver correlated to extrahepatic tumor signaling.
From: SPP1 + macrophages cause exhaustion of tumor-specific T cells in liver metastases
AH1 + CD8 + T cells in the liver of subcutaneous tumor-bearing mice expressing CD39 (A; day 7 vs 14 p = 0.0083; day 7 vs 21 p < 0.0001; day 14 vs 21 p < 0.0001), TIM3 (B; day 7 vs 14 p = 0.0760; day 7 vs 21 p < 0.0001; day 14 vs 21 p < 0.0001), PD1 (C; day 7 vs 14 p < 0.0001; day 7 vs 21 p < 0.0001; day 14 vs 21 p < 0.0001), and CD69 (D; day 7 vs 21 p < 0.0001; day 14 vs 21 p < 0.0001). E Correlation of fibronectin expression in metastatic intrahepatic tumors (MT) to TGF-β and IL-6 in primary tumors (n = 10). F Correlation of fibronectin expression in primary CRC tumors (PT) to TGF-β and IL-6 in primary tumors (n = 10). G Correlation of SPP1 expression by macrophages in metastatic intrahepatic tumors (MT) to TGF-β and IL-6 expression in primary tumors (n = 10). H Correlation of SPP1 expression by macrophages in primary CRC tumors to TGF-β and IL-6 in primary tumors (n = 10). Data was analyzed with simple linear regression (E–H) and one-way Anova (A–D): *P < 0.05; **P < 0.01; ***P < 0.005; ****P < 0.001, ns = not significant.
