Fig. 10: HypnoS revealed ENT1/2 mediate Ado transportation during sleep-wake cycles. | Nature Communications

Fig. 10: HypnoS revealed ENT1/2 mediate Ado transportation during sleep-wake cycles.

From: A high-performance fluorescent sensor spatiotemporally reveals cell-type specific regulation of intracellular adenosine in vivo

Fig. 10

a Schematic diagram depicting the strategy for fiber photometry recording of HypnoS fluorescence in neurons in the BF of wild-type (WT) or ENT1/2 double knockout (dKO) mice during the sleep-wake cycle. b EEG power spectrogram, EMG (scale, 2 mV), and HypnoS recordings (scale, 20 z-score) in WT (bottom) or ENT1/2 dKO (top) mice. In this and subsequent figures, the wake state is shaded in blue, REM sleep is shaded in pink, and NREM sleep is unshaded. c Time course for the HypnoS sensor measured in neurons in WT (green; n = 10 sessions from 8 mice) or ENT1/2 dKO (bule; n = 7 sessions from 4 mice) mice during the indicated transitions between sleep-wake states. The vertical dashed lines at time 0 represent the transition time. d Summary of the average HypnoS fluorescence responses measured in neurons in WT (green; n = 10 sessions from 8 mice) and ENT1/2 dKO (blue; n = 7 sessions from 4 mice) mice in the indicated sleep-wake states. Each symbol represents data from one recording. Two-tailed Student’s t-test: for wake, P = 0.0023; for NREM, P = 0.1204; for REM, P = 0.0020 between WT and ENT1/2 dKO mice. e–h Similar to a–d except for astrocyte expressing HypnoS. g Time course for the HypnoS sensor measured in astrocytes in WT (green; n = 8 sessions from 3 mice) or ENT1/2 dKO (blue; n = 8 sessions from 4 mice) mice during the indicated transitions between sleep-wake states. h Summary of the average HypnoS fluorescence responses measured in astrocytes in WT (green; n = 7 sessions from 3 mice) and ENT1/2 dKO (blue; n = 8 sessions from 4 mice) mice in the indicated sleep-wake states. Each symbol represents data from one recording. Two-tailed Student’s t-test: for wake, P = 0.000040; for REM, P = 0.00052; for NREM, P = 0.0078 between WT and ENT1/2 dKO mice. Data are shown as mean ± s.e.m. in (c, d, g, h) with the error bars or shaded regions indicating the s.e.m. **P < 0.01; ***P < 0.001; ns not significant. Source data are provided as a Source Data file.

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