Fig. 2: Identification of exclusive ecGenes in rice during optimal growth.
From: Adaptive dynamics of extrachromosomal circular DNA in rice under nutrient stress
a Count distribution of identified exclusive ecGenes between 1-day control samples (Ctrl_D1) and 3-day control samples (Ctrl_D3). b Count distribution of identified exclusive ecGenes between 1-day control samples (Ctrl_D1) and 7-day control samples (Ctrl_D7). c Count distribution of identified exclusive ecGenes between 1-day control samples (Ctrl_D1) and 14-day control samples (Ctrl_D14). d Dot-plot illustrating the significantly enriched GO biological process categories summarized by REVIGO for the exclusive ecGenes during optimal growth from Ctrl_D1 vs. Ctrl_D3, Ctrl_D1 vs. Ctrl_D7, and Ctrl_D1 vs. Ctrl_D14. e Karyoplots of the Chr10:15806500-15811000 locus showing (from top to bottom) regions of eccDNAs and ONT sequencing coverage in two replicates of 14-day control samples (Ctrl_D14_1 and Ctrl_D14_2), regions of SANGER sequencing (SANGERseq) fragments from inverse PCR validation in 14-day control samples, and the transcript structure of Os10g0439924. f Gel electrophoresis of inverse PCR products from the ecGene derived from the Os10g0439924 locus in 14-day control samples (Ctrl_D14). The experiment was conducted twice. M: DNA Marker; bp: base pair; gDNA: template genomic DNA from Ctrl_D14; RCA: template rRCA products from Ctrl_D14; forward primer: 5’-CCCTACATATTGCCCATGCATTCAC-3’; reverse primer: 5’-GTATTGGAAGAGTGCCGAGGAG-3’. Circles in red: symbol used for pointing out amplified products of ecGene.
