Fig. 6: Park7 ablation exaggerates BAT whitening at thermoneutrality.

a–c Histochemistry analysis of BAT of male Park7^ΔUcp1 and Flox mice housed at TN for 8 weeks, including hematoxylin-eosin staining (a), immunohistochemical staining of UCP1 (b) and F4/80 (c). Arrow indicated crown-like structure. Scale bars, 20 µm. d Representative EM images of BAT from male Park7^ΔUcp1 and Flox mice housed at TN for 8 weeks. Scale bars, 2 µm. e Immunoblotting for mitochondrial proteins in BAT from male Park7^ΔUcp1 and Flox mice housed at TN for 8 weeks (n = 3 mice per group). f Oxygen consumption rate (OCR) analysis of BAT from male Park7^ΔUcp1 and Flox mice housed at TN for 8 weeks (n = 4). g–i RNA-seq analysis of BAT from male Park7^ΔUcp1 and Flox mice housed at TN for 8 weeks, including heatmap of all changed genes (g), GO pathway analysis of up-regulated pathways (h), heatmap of whitening, thermogenesis, inflammation, fatty acid oxidation genes (i). j QPCR analysis for mRNA levels of Pparγ, Acadm, Ucp1, Ppargc1a, Dio2, Pank3 of BAT from male Park7^ΔUcp1 and Flox mice housed at TN for 8 weeks (n = 5). k Immunoblotting for protein levels of PPARγ, PPARα, CPT1α, CPT1β, PGC1α, UCP1 of BAT from male Park7^ΔUcp1 and Flox mice housed at TN for 8 weeks and quantification (n = 3 mice per group). Statistical significance was assessed by unpaired Student’s t-test. All results were expressed as means ± SEM and *p < 0.05, **p < 0.01. Student’s two-tailed unpaired T-test for 2-group comparisons. Source data are provided as a Source Data file.