Fig. 5: In vitro and in vivo experiments for NIR fluorescence imaging through adipose tissue.
From: High-contrast in vivo fluorescence imaging exploiting wavelengths beyond 1880 nm
a A bright-field image showing two capillaries loaded with hybrid QDs fluorescent probes and buried in the porcine adipose tissue. b Fluorescence images of capillaries through adipose tissue of 2 mm thickness within 900–1100, 1100–1300, 1300–1400, 1400–1500, 1500–1700, and 1700–2080 nm windows. c Fluorescence images of capillaries through adipose tissue of 1, 2, and 3 mm thickness at the 1700–2080 nm window. d Normalized fluorescence intensity profiles along the red dashed lines in (b). e SBR analysis of the profiles in (d). f FWHM analysis of the profiles in (d). g, h Fluorescence images of rabbit bile ducts without adipose tissue covered in the 1700–2080 nm window. Scale bars of (g): 5 mm. CHD common hepatic duct, CBD common bile duct. i Normalized fluorescence intensity profile along the yellow dashed line in (g) and the corresponding Gaussian-fitted FWHM value. j, k Fluorescence images of rabbit bile ducts covered with porcine adipose tissue of ~2 mm thickness in the 1700–2080 nm window. Scale bars of (j): 5 mm. l Normalized fluorescence intensity profile along the yellow dashed line in (j) and the corresponding Gaussian-fitted FWHM value, where the dots represent the raw data and the blue line represents Gaussian fitted curves.
