Fig. 3: Cadherins drive cell-type- and input-specific inhibition onto L5 pyramidal cell populations. | Nature Communications

Fig. 3: Cadherins drive cell-type- and input-specific inhibition onto L5 pyramidal cell populations.

From: Cadherins orchestrate specific patterns of perisomatic inhibition onto distinct pyramidal cell populations

Fig. 3

a, e Conditional viral strategy to down-regulate Cdh12 and Cdh13 in L5 IT and L5 ET neurons and measure its impact on perisomatic inputs at P30. b, f Representative 3D-reconstructed images of PV (SYT2+) and CCK inputs (CB1R+) onto L5 IT and L5 ET somas. c SYT2+ boutons density onto L5 IT (Control, n = 4 mice, 94 cells; Cdh12 KD, n = 6 mice, 140 cells; Cdh13 KD, n = 4 mice, 119 cells; One-way ANOVA p = 0.1136, Tukey’s posthoc: Control vs Cdh12 KD ns, Control vs Cdh13 KD ns, Cdh12 KD vs Cdh13 KD ns). d CB1R+ boutons density onto L5 IT (Control, n = 4 mice, 95 cells; Cdh12 KD, n = 6 mice,141 cells; Cdh13 KD, n = 4 mice, 107 cells; One-way ANOVA ***p = 0.0002, Tukey’s posthoc: Control vs Cdh12 KD **p = 0.0017, Control vs Cdh13 KD p = 0.2888, Cdh12 KD vs Cdh13 KD ***p = 0.0002). g SYT2+ boutons density onto L5 ET (Control, n = 11 mice, 291 cells; Cdh12 KD, n = 8 mice, 153 cells; Cdh13 KD, n = 8 mice, 170 cells; One-way ANOVA *p = 0.0143, Tukey’s posthoc: Control vs Cdh12 KD p = 0.8291, Control vs Cdh13 KD *p = 0.0401, Cdh12 KD vs Cdh13 KD *p = 0.0180). h CB1R+ boutons density onto L5 ET (Control, n = 11 mice, 220 cells; Cdh12 KD, n = 7 mice, 133 cells; Cdh13 KD, n = 8 mice, 141 cells; One-way ANOVA p = 0.2959, Tukey’s posthoc: Control vs Cdh12 KD ns, Control vs Cdh13 KD ns, Cdh12 KD vs Cdh13 KD ns). Data are represented as mean ± SEM, *p < 0.05; **p < 0.01; ***p < 0.001; ns not significant. Scale bars: 10 μm (b, f). Source data are provided as a Source Data file.

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