Fig. 6: LptM translocates across the OM in an ATP- and LPS- dependent manner.
From: Surface lipoprotein sorting by crosstalk between Lpt and Lol pathways in gram-negative bacteria
A In vitro membrane-to-membrane transport assays showing that LptM translocates across the OM in an ATP- and LPS- dependent manner. Four different types of nanodiscs were prepared for the assays: LptB2FGC-A, LptB2FGC-A+LptM, LptB2FGC-A + LPS and LptDI230pBpaE-LptMStrep. The LptD×LptM crosslinks decreased in an ATP- and time- manner only when nanodisc-embedded LptDI230pBpaE-LptMStrep was incubated with the nanodisc containing both LptB2FGC-A and LPS. Cartoons show experimental designs of the in vitro LPS transport assays. Data are representative of three independent experiments. B The proposed model of SLP transport to the cell surface. The red dash line indicates that LptA crosstalks to the Lol pathway during OM biogenesis. Source data are provided as a Source Data file.
