Fig. 2: Increased endogenous or exogenous FABP4 protein does not affect the osteogenic differentiation of BMSCs.

a Full image of the effect of rmFABP4 (25 ng/mL or 50 ng/mL) or combined with FABP4 inhibitor BMS (5 μM or 10 μM) on BMSCs osteogenic differentiation, assessed by Alizarin Red staining. b Quantitative analysis of (a). n = 6 wells in 6-well-plate per group. c Representative bright-field and GFP images of FABP4 stably overexpressed BMSCs. Scale bar, 200 μm. At least three independent experiments were performed. d Western Blotting indicated the successful overexpression of FABP4 protein in FABP4 overexpressed BMSCs. Three independent experiments were performed. e q-PCR results showed a nearly 3600-fold increase in FABP4 expression in BMSCs. n = 3 independent samples per group. f Full image of the effect of FABP4 overexpressed BMSCs osteogenic differentiation by Alizarin Red staining. g Quantitative analysis of (f). n = 6 wells in 6-well-plate per group. All experiments were run in at least triplicate. All data is presented as mean ± SEM with p-values (b, e, g), ns = no significance. Two-tailed unpaired t test (e) and one-tailed Dunnett’s Multiple Comparison Test (b, g) were performed, and the p-values were adjusted made by multiple comparisons (b, g). Source data are provided as Source Data file.