Fig. 7: Click chemistry constructs NPs for bone-targeted delivery of FABP4 inhibitors. | Nature Communications

Fig. 7: Click chemistry constructs NPs for bone-targeted delivery of FABP4 inhibitors.

From: FABP4 inhibition suppresses bone resorption and protects against postmenopausal osteoporosis in ovariectomized mice

Fig. 7

a Systematic strategy for one-step click chemistry synthesis of PLGA-PEG-Ald amphiphilic polymers. b Description of the self-assembly process of polymers into Ald-BMS-NPs. c Zeta potentials of PLGA-BMS-NPs and Ald-BMS-NPs. Data was represented mean ± SEM with p values; n = 3 independent samples per group. d DLS size distribution and TEM morphology of Ald-BMS-NPs. Scale bar, 100 nm. e DLS size distribution and TEM morphology of PLGA-BMS-NPs. Scale bar, 100 nm. Data was represented mean ± SEM; n = 3 independent samples per group. f Cumulative release curve of BMS in pH 7.0 PBS over time for PLGA-BMS-NPs and Ald-BMS-NPs. Data represent mean ± SEM; n = 3 independent samples per group. g In situ fluorescence images of BMMs uptake of Ald-BMS-Cy5-NPs and PLGA-BMS-Cy5-NPs after 30 min. Scale bar, 10 μm. h Representative TEM images of surface-bound nano-hydroxyapatite on Ald-NPs and PLGA-NPs. Scale bar, 200 nm. All experiments were run in triplicate. Data was analyzed by two-tailed unpaired t test (c) and one-sample t-test (d–f). Source data are provided as Source Data file.

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