Fig. 1: Knock-down of AeDHPAAS in female adult mosquitoes.

a AeDHPAAS transcript abundance after RNAi was detected by qPCR. Twelve mosquitoes were analyzed per group, and the experiment was replicated three times. DEPC 95% confidence interval (CI) = 1.00–1.00; GUS 95% CI = 0.93–1.18; DS 95% CI = 0.26–0.33. DEPC vs GUS, Q = 3.04, degrees of freedom (df) = 6, P = 0.14; DEPC vs DS, Q = 40.30, df = 6, P < 0.001. b Female mosquito survival, 12 h after injection with Ringer’s solution following RNAi. 100 mosquitoes were analyzed from each group, and the experiment was replicated three times. DEPC 95% CI = 92.93–104.40; GUS 95% CI = 89.08-104.26; DS 95% CI = 27.09-56.90. DEPC vs GUS, Q = 0.84, df = 6, P = 0.42; DEPC vs DS, Q = 23.88, df = 6, P < 0.0001. c Mosquito weight after RNAi and before blood meal. 15 mosquitoes were analyzed from each group, and the experiment was replicated ten times. DEPC 95% CI = 1.57–1.91; GUS 95% CI = 1.56–1.90; DS 95% CI = 1.65-2.07. DEPC vs GUS, Q = 0.12, df = 27, P = 0.93; DEPC vs DS, Q = 1.49, df = 27, P = 0.32. d Blood meal weight after RNAi. 15 mosquitoes were analyzed from each group, and the experiment was replicated 10 times. DEPC 95% CI = 3.50-4.42; GUS 95% CI = 3.23–4.00; DS 95% CI = 1.88–2.67. DEPC vs GUS, Q = 2.02, df = 27, P = 0.21; DEPC vs DS, Q = 10.67, df = 27, P < 0.0001. X-axes represent different treatment groups, and y-axes represent relative AeDHPAAS expression (a), survival rate (b), mosquito weight (c), and blood meal weight (d). Data were presented as mean values ± standard deviation (SD). Ordinary one-way analysis of variance (ANOVA) with two-sided Tukey’s multiple comparisons test was used. *** indicates P < 0.001; **** indicates P < 0.0001; ns indicates no significance. Source data are provided as a Source Data file. DEPC mosquitoes treated with diethylpyrocarbonate (DEPC)-treated water, GUS mosquitoes treated with gus‑dsRNA, DS mosquitoes treated with AeDHPAAS‑dsRNA.