Fig. 4: TEM analysis of adult cuticular structure after RNAi knock-down of AeDHPAAS. | Nature Communications

Fig. 4: TEM analysis of adult cuticular structure after RNAi knock-down of AeDHPAAS.

From: 3,4-Dihydroxyphenylacetaldehyde synthase evolved an ordered structure to deliver oxygen to pyridoxal 5’-phosphate for cuticle assembly in the mosquito Aedes aegypti

Fig. 4

a Natural cuticular structure after DEPC water injection. b Negative control cuticular structure after gus‑dsRNA injection. c Experimental cuticular structure after AeDHPAAS‑dsRNA injection. The scale bar in each image represents 1 µm. d, e Analysis of endocuticular layer and exocuticular layer thickness, based on the scale of three TEM samples per group. Three mosquito cuticles were analyzed from each group. Data were presented as mean values ± SD. An ordinary one-way ANOVA with two-sided Tukey’s multiple comparisons test was used for statistical analysis. d DEPC 95% CI = 1.04–1.75; GUS 95% CI = 1.15–1.63; DS 95% CI = 0.04–1.15. DEPC vs GUS, Q = 0.04, df = 6, P = 0.97; GUS vs DS, Q = 8.48, df = 6, P < 0.01; DEPC vs DS, Q = 8.51, df = 6, P < 0.01. e DEPC 95% CI = 0.29-1.05; GUS 95% CI = 0.60-0.89; DS 95% CI = 0.86-1.67. DEPC vs GUS, Q = 0.93, df = 6, P = 0.49; GUS vs DS, Q = 6.80, df = 6, P < 0.01; DEPC vs DS, Q = 7.74, df = 6, P < 0.01. ** indicates P < 0.01; ns indicates no significant difference. Source data are provided as a Source Data file. exo exocuticular layer, endo endocuticular layer, DEPC mosquitoes treated with DEPC water, GUS mosquitoes treated with gus‑dsRNA, DS mosquitoes treated with AeDHPAAS‑dsRNA.

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