Fig. 3: Elucidation of the potential AKRs involved in the diol synthesis.
From: Microbial synthesis of branched-chain β,γ-diols from amino acid metabolism
a dCas12-mediated CRISPRi strategy to repress the expression of AKR genes (YdjG, YdbC, YgdS, YghZ, and YdhF). b The distribution profile of the product 4-M-PDO (1c) and the intermediate 3-H-4-MP-2-one (1b). Reactions were carried out by strains carrying pRSF-Ilv2c and different CRISPRi inhibitory plasmids. The star sign indicates obvious observation of product distribution changes. Data represent the mean value ± SD from three biological replicates. The product distribution profile obtained by GC-FID analysis shows the enzyme activity of YdjG (c), YghZ (d) and YdhF (e). Experiments were conducted using the substrates of 3-H-4-MP-2-one and 4-M-PDO obtained from biocatalysis. For each reaction, 5 mg/mL of purified proteins was individually added to the biocatalytic system. Experiments were carried out in three biological repeats, and representative results are shown. Source data are provided as a Source Data file.
