Fig. 3: Microextraction of glycosphingolipids in human serum.

a Schematic overview of serological glycosphingolipids (GSLs) extraction method; b Profiled coverage of sialylated GSLs in four-fraction extract and two-fraction extract (Supplementary Data 2 and 3); c Partition of internal standards calculated based on absolute intensity of each internal standard between two fractions in inter-day extract replicates of reference serum (n = 4). 300 µL of reference serum from volunteers was used for each extraction. Source data are provided as a Source Data file.