Fig. 4: Representative PASEF spectra of glycosphingolipids in human serum.

a Baseline separation of Neu5Ac-nLc4Cer 18:1;O2/18:0 and GM1 18:1;O2/18:0 in extracted ion mobilogram at m/z 794.93; b PASEF spectra of GQ1b 18:1;O2/18:0; c PASEF spectra of Neu5Ac-nLc6Cer 18:1;O2/14:0; d PASEF spectra of HexNAcHex3Cer 18:1;O2/16:0; e PASEF spectra of SHex2Cer 18:1;O2/16:0. PASEF spectra were acquired using a collision energy of 50 eV. Blue circle represents Glucose, yellow circle represents Galactose, yellow square represents N-acetylgalactosamine, purple diamond represents N-acetylneuraminic acid, and red triangle represents Fucose.