Fig. 6: The distribution of HSYA-related genes in plants.
From: Elucidation of the biosynthetic pathway of hydroxysafflor yellow A
a LC/MS analysis of Nicotiana benthamiana leaves infiltrated with different combinations of genes, showing extracted ion chromatograms (EIC: m/z 611.1618) of HSYA. The peak intensity is shown on the EIC plot. b The competing biosynthesis pathways of HSYA (quinochalcone C-glycoside) and flavone C-glycosides. c, d Homology analysis of Ct2OGD1 and CtF6H homologous genes from 19 plant species of the ONEKP database (https://db.cngb.org/onekp/). Some genes were not complete in four species (Q. Shumardii, D. macropodum, F. cronquestii, and S. reclinatum). e LC/MS analysis and expression level analysis of C. tinctorius (red flower) and its variant (white flower). The peak with a retention time similar to that of HSYA in the white flowers was not identified as HSYA, according to mass spectral analysis. n = 3, three biologically independent samples were tested; The data were presented as mean values ± SEM. The source data underlying Fig. 6e are provided in a Source Data file.
