Fig. 2: Identification of pepstatins and the pep biosynthetic gene cluster.
From: Tandem ketone reduction in pepstatin biosynthesis reveals an F420H2–dependent statine pathway
a UPLC-HRMS analysis (base peak chromatogram (BPC)) of pepstatin congeners (1-4) produced by Streptomyces catenulae DSM40258 (i); Knockingout pepD abolished pepstatin 1-4 production (ii); Pepstatin congeners (1-4) produced by heterologous expression of BGC pep in Del14 (Del14-pep) (iii); The production of 1-4 significantly decreased by pepJ deletion in Del14-pep-ΔpepJ (iv); The production of 1-4 increased in Del14-pep-pepJ-act by promoter exchange of pepJ (v). b The chemical structures of pepstatins isolated from Streptomyces catenulae DSM40258. c The schematic representation of the pepstatin BGC from S. catenulae DSM40258.
