Fig. 4: Spatial distribution of stromal and tumor in Diffuse Midline Glioma A1.
a Graph clustering assignment spatial map of Diffuse Midline Glioma A1, an IDH wild-type, EGFR negative tumor. b Boxplots of ESTIMATE stromal scores for each cluster. The minima and maxima are noted by the whiskers for each boxplot. The 25th percentile and 75th percentile are shown as the bounds of each box. A line across each box represents the median value. The minima is calculated as Q1-1.5*(interquartile range), and the maxima is calculated as Q3 + 1.5*(interquartile range). The mean for each cluster is shown as a point on each boxplot. Mean, median, and number of observations are displayed above each plot. All data points are shown behind each boxplot. c ESTIMATE tumor purity spatial overlay. d ESTIMATE stromal score spatial overlay. e PDGFRA log normalized counts spatial overlay. f Copies of chromosome 10 per spot, whereby the average of normalized gene expression for all genes above the average of 0.1 counts, where tumor regions are expected to have chromosome 10 deleted. g tLOH output of allele fractions for SNPs with total read counts greater than 20. Points are highlighted based on state determination, where blue is heterozygous, and gold is LOH.
