Fig. 3: Vaccine-induced antibodies and protection of CAST mice against a lethal MPXV challenge. | Nature Communications

Fig. 3: Vaccine-induced antibodies and protection of CAST mice against a lethal MPXV challenge.

From: Mpox multiprotein virus-like nanoparticle vaccine induces neutralizing and protective antibodies in mice and non-human primates

Fig. 3: Vaccine-induced antibodies and protection of CAST mice against a lethal MPXV challenge.

a CAST mice (n = 10) per group, combined from two experiments, were immunized IM three times at 3-week intervals and bled prior to each immunization. Mice were challenged IN with 104 PFU of clade IIa MPXV at 3 weeks after the third immunization. b Anti-VACV and c anti-MPXV neutralization titers were determined in duplicate in individual mice prior to immunization (Pre-bleed) or at 3 weeks after receiving 1 (Prime), 2 (Boost), or 3 (2nd Boost) IM immunizations with 2 µg of M1 VLP; a combination of 2 µg each of M1 + A35 + B6 VLPs; or 107 PFU of MVA. Bars are geometric means. Significance was determined with one-way ANOVA and a multiple comparison test. d Weight loss and e survival of individual CAST mice challenged IN with 106 PFU of MPXV clade IIa at 3 weeks after third immunization with indicated VLPs, SPs, MVA, or control unconjugated SC-mi3. Due to accidental loss, there were 9 immunized mice in the A35 VLP, B6 VLP, and MVA; 8 in the M1 + A35 + B6 VLPs; 7 in the M1 VLPs; and 6 in the SC-mi3 groups. Bars are SD. Source data are provided as a Source Data file. Created in BioRender. Moss (2025) https://BioRender.com/xkyulng.

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