Fig. 4: Reduction of VACV replication at the IN site of inoculation and spread. | Nature Communications

Fig. 4: Reduction of VACV replication at the IN site of inoculation and spread.

From: Mpox multiprotein virus-like nanoparticle vaccine induces neutralizing and protective antibodies in mice and non-human primates

Fig. 4: Reduction of VACV replication at the IN site of inoculation and spread.

a BALB/c mice (n = 4 per group) received 1 (1×) or 2 (2×) IM immunizations with a mixture of M1, A35, and B6 VLPs or SPs, MVA, or SC-mi3 control and challenged 3 weeks later IN with 106 PFU of WRvFire. Weight loss and survival were determined. Bars represent SD. b Mice in (a) were imaged following IP injection of luciferin on indicated days. BLI is depicted by a pseudocolor scale with intensity increasing from blue to red. c, d Photon flux measurements of mice from (a). Regions of interest were drawn to distinguish luminescence from the head (c) and from the body (chest and abdomen) (d). Total photon flux is plotted as photon/s/cm2/sr. Symbols: †, death due to infection; *, anesthesia death. Significance between control SC-mi3 (combined 1× and 2×) and vaccine-immunized mice determined for day 6 by the Kruskal–Wallis/Dunn’s multiple comparison test. Source data are provided as a Source Data file.

Back to article page