Fig. 3: Perfusion data characterising the intervention.

For subplots (A–C), the black line shows the mean value for all cases perfused to that time point; the shaded area shows standard deviation. Horizontal red dashed lines indicate normal physiological ranges. For subplots (D–I), points indicate means, error bars standard deviations. A Renal blood flow during perfusion. B Oxygen tension during perfusion. C Arterial pH during perfusion (normal range 7.35–7.45). D Sodium concentration during perfusion (normal range 135–145 mmol/L). E Osmolality during perfusion (normal range 275–295 mosmol/L). F Ionised calcium during perfusion (normal range 1.16–1.31 mmol/L). G Perfusate albumin concentration during perfusion (normal range 35–55 g/L). H Perfusate glucose and lactate concentrations during perfusion (glucose normal range 3.5–7.8 mmol/L). I Perfusate insulin concentration during perfusion. For subplots (A–I) data are shown up to 12 h only, due to the small number of kidneys perfused for greater than 12 h (number of kidneys per time-point: 0 and 2 h, n = 36; 4 h, n = 27; 6 h, n = 16; 8 h, n = 11; 10 h, n = 6; 12 h, n = 4). J A summary chart showing the total preservation durations, divided into cold ischaemic (CIT) and normothermic machine perfusion (NMP) times. The maximum NMP duration was 23.4 h, resulting in an overall preservation time of 37.3 h. Source data are provided as a Source Data File.