Fig. 4: Biomarkers.

A Vascular resistance (adjusted for the weight of the kidney) during the first 2 h of perfusion, stratified by early functional status (DGF Delayed Graft Function, PGF Primary Graft Function), and showing the profile of the single kidney which was discarded based on perfusion parameters. Solid lines indicate group means; shaded areas indicate mean ± SD. B Weight-adjusted vascular resistance compared between kidneys from donors with a history of hypertension, and without (Wilcoxon signed-rank test, two-sided, n = 36). C Weight-adjusted vascular resistance compared between kidneys with histopathological evidence of donor vascular disease, and without (Wilcoxon signed-rank test, two-sided, n = 36). D Ex-situ renal function (urine output shown; see Supplementary Information for creatinine clearance and fractional excretion of sodium) was not significantly different between the functional DGF (fDGF) and PGF groups (Wilcoxon signed-rank test, two-sided, n = 36). E Hour 2 perfusate NGAL concentration was not significantly different between the fDGF and PGF groups, however perfusate LDH, GST-Pi, L-FABP, and IL-18 (subplots F, G, H, I) all showed significant differences (T-tests other than L-FABP, Wilcoxon signed-rank test, all n = 35, all two-sided). For GST-Pi in particular the difference was large and highly statistically significant, even after adjustment for multiple comparisons. Boxplots B-I: centre lines shown are medians; bounds of the boxes are first and third quartiles; whiskers extend to either the most extreme value or the limit of the box ± 1.5x IQR, whichever is closest to the median; individual datapoints are overlaid and jittered. J Relationship between the change in GST-Pi concentration observed in perfusate during perfusion, and post-transplant renal function (linear regression, Pearson’s correlation coefficient shown, n = 35, shaded area indicates 95% CI). This association was preserved out to 12 months and remained significant after adjustment for multiple comparisons. Source data are provided as a Source Data File.