Fig. 1: Characteristics of study participants and experimental design.
a Distribution of total HIV DNA in Fiebig stage III participants in RV254 at week 48 after ART initiation and their categorization into three groups based on reservoir size. The center of the box plot represents the median (50th percentile), while the upper and lower bounds of the box represent the lower and upper quartiles (25th and 75th percentiles, respectively). The whiskers extend from the box to the data points at most 1.5 times the interquartile range (IQR) from the lower and upper quartiles. b Selected participants from Fiebig stage III with extreme reservoir size phenotypes (undetectable = below LOD; and detectable = high) of cell-associated total HIV DNA in the RV254 Thai discovery cohort (n = 14). Significance was determined by the Mann-Whitney U two-sided test. c Total HIV DNA decay between weeks 0 (AHI) and 48 (after ART initiation). Significance was determined by the Wilcoxon signed-rank two-sided test. d Phenotypes of participants comprising the detectable versus undetectable reservoir size categories. Mean values are shown for each group, NS not significant. Significance was determined by the Mann-Whitney U two-sided test. e Single-cell RNA-seq and multiparameter flow cytometry were performed on all 14 participants. Additional validation by scRNA-seq was performed in an independent AHI cohort from the USA (A5354) (n = 38).
