Table 1 Cumulative numbers of sulfatide subclass isoforms identified in ARSA−/− mouse kidney by LC-MS or MALDI-MSI using various analytical workflows

From: Deep MALDI-MS spatial omics guided by quantum cascade laser mid-infrared imaging microscopy

    

SM4

SM3

SM2a

SM1b

SM1a

SB1a

total

LC-TIMS-MS

timsTOF-MS

60w_1

41

25

0

0

0

26

92

60w_2

42

26

0

0

0

27

95

12w_1

25

14

0

0

0

4

43

12w_2

33

20

0

0

0

11

64

timsTOF-MS2

60w_1

38

25

0

0

0

26

89

60w_2

39

26

0

0

0

27

92

12w_1

22

14

0

0

0

4

40

12w_2

30

20

0

0

0

11

61

MALDI timsTOF MSI

qTOF mode

all

60w_1

44

30

4

13

10

101

60w_2

44

33

5

17

13

112

12w_1

34

21

2

11

9

77

12w_2

30

25

1

9

9

74

“clean”

60w_1

15

16

2

10

4

47

60w_2

14

22

2

12

5

55

12w_1

11

13

1

8

4

37

12w_2

10

16

1

6

3

36

MIR-guided

all

60w_1

60

31

6

20

17

21

155

60w_2

62

31

6

20

17

20

156

12w_1

52

26

6

13

11

12

120

12w_2

51

26

6

14

12

17

126

“clean”

60w_1

40

19

6

17

17

16

115

60w_2

44

23

6

15

17

14

119

12w_1

34

15

6

13

11

11

90

12w_2

36

12

6

13

12

12

91

  1. Whole kidney sections of 12- or 60-week-old ARSA−/− mice (n = 2 each) were analyzed by LC-TIMS-MS or –MS2 and MALDI-MSI (TIMS/qTOF). “Clean” peaks fulfilled higher quality standards, defined as peaks that do not feature at least one other peak within a mass window of ±1.1 Da (qTOF mode MSI) and a mobility window of ±0.005Vs/cm2 (TIMS-MSI), and were manually curated. QCL-MIR imaging-guided MSI focused on the kidney’s ISOM and IMP ROIs only.
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