Fig. 5: Sequential HS-AFM images of ADAR1 dimer interacting with substrate dsRNA.
From: High-speed atomic force microscopy and 3D modeling reveal the structural dynamics of ADAR1 complexes
HS-AFM direct evidence of searching, recognizing, capturing, and scanning dsRNA by ADAR1 dimers. An ADAR1 dimer was observed at different sites and formed the ternary complex. The images show that ADAR1 utilizes N-terminal domains for searching, recognizing, and capturing dsRNA, and then the deaminase domain dimer loops out on the dsRNA for scanning. Imaging parameters: scanning area = 150 × 150 nm2 (100 × 100 pixels); frame rate = 5 frames/s.
