Fig. 2: Centromeres are methylated slower and reach a limit, unlike other genomic regions.
From: Nucleosome dynamics render heterochromatin accessible in living human cells
a Heat map showing the variation in methylation rate at the chromosomal level. The average methylation rate was calculated for all GATC sites in each 100 kb window in the T2T genome by plotting ‘ln (1- fraction methylated)’ against time after adenovirus transduction, and then normalised to the genomic average rate to obtain relative rates. Red rectangles: centromeric regions. b Schematic of the organisation of the various centromeric elements defined in the T2T genome assembly (adapted from ref. 37). Divergent α-Sat and monomeric α-Sat were combined as “α-Sat other”. c Median GATC methylation time courses and d, methylation rates for the various centromeric elements. e, Median GATC methylation time courses and f, methylation rates for the various active supra-chromosomal α-satellite families.
