Fig. 5: Dynamics of acetate consumption within colony.
a–b The spatial concentration profile of acetate (mM) represented by a colormap, in the colony and the portion of the agar region below the colony for the simulation with 20 mM initial glucose concentration, at 15 h (a), and 30 h (b) of colony development. c Acetate concentration (mM) along the central vertical axis of the simulated colony plotted against the distance (μm) from colony bottom at 10 h, 12 h, 15 h 20 h and 40 h stages of colony development (time coded by color). The grey shaded region represents concentrations below the Monod constant. d Glucose (yellow), oxygen (blue), and acetate (salmon) concentration normalized by respective Monod constants Kg, Ko, and Ka along the central vertical axis of the colony plotted against the vertical distance from colony bottom (μm) at 30 h stage of colony development. The grey shaded region indicates the concentration falling below the Monod constant. e The dependence of EQ59 cell growth rate (h−1) on acetate concentration during batch culture growth with acetate as the sole carbon source (open circles). The solid line represents the fit based on a Lineweaver-Burk plot of data (Fig. S10a, b) and grey shaded region represents the concentration regime below the Monod constant (~5 mM) obtained using the fit. f, g Spatial profile of cell growth rate (h−1) arising from acetate metabolism (coded by color) within a 15 h (f), and 30 h (g) old colony for simulation with 20 mM initial glucose concentration. h The percentage of the acetate uptake flux (integrated over entire colony) towards cell growth (light blue) and towards maintenance (green) plotted against time (h) of simulated colony development. i, j Spatial distribution of acetate uptake rate for cell maintenance in mmol/gdw/h units (coded by color) within a 15 h (i), and 30 h (j) old colony for simulation with 20 mM initial glucose concentration.
