Fig. 2: Differential abundance analysis on PBMCs derived from SSc patients with scleroderma renal crisis and interstitial lung disease. | Nature Communications

Fig. 2: Differential abundance analysis on PBMCs derived from SSc patients with scleroderma renal crisis and interstitial lung disease.

From: Single-cell analysis reveals immune cell abnormalities underlying the clinical heterogeneity of patients with systemic sclerosis

Fig. 2: Differential abundance analysis on PBMCs derived from SSc patients with scleroderma renal crisis and interstitial lung disease.

a Neighborhood graph of PBMCs from SSc patients, generated using Milo differential abundance testing. Nodes represent neighborhoods of the PBMCs. The color scale indicates the log2-fold difference between SRC patients and the other SSc patients. Neighborhoods showing an increase in SRC are colored in red, while those with a decrease are in blue. b Beeswarm and box plots showing the distribution of log2-fold differences between SRC patients (n = 4) and the other SSc patients (n = 17) in neighborhoods in different cell subsets colored as in (a). Box plots show median and interquartile range (IQR); the lower and upper hinges correspond to the first and third quartiles. The upper whisker extends from the hinge to the largest value that is no further than 1.5 × IQR from the hinge. The lower whisker extends from the hinge to the smallest value that is at most 1.5 × IQR from the hinge. Neighborhood graph (c) and Beeswarm plots (d) of PBMCs from ILD without SRC (n = 10) and the other SSc patients (n = 11), generated in a similar fashion as in (a) and (b), respectively. Neighborhoods showing an increase in ILD without SRC are shown in red, while those with a decrease are in blue.

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