Fig. 2: Sub-cellular and sub-ring localisation of CdpA with FtsZ1 or FtsZ2.
From: Cell division protein CdpA organises and anchors the midcell ring in haloarchaea
a Phase-contrast and fluorescence images of H. volcanii strains YL6 (H26 + CdpA-GFP + FtsZ1-mCherry dual expression) and YL7 (H26 + CdpA-GFP + FtsZ2-mCherry dual expression) cultured with 0.2 mM Trp during mid-log growth. The middle graphs show the intensity profile of the fluorescence signal (magenta for mCherry, and green for GFP) by plotting the normalized median intensity perpendicular to the long axis of cells. The right graphs show correlation coefficients plotted as a frequency distribution. n = number of cells examined in one independent experiment. The data shown are representative of at least two independent experiments. Scale bars, 5 µm. Source data are provided as a Source Data file. b Dual-colour of 2D-SIM images of mid-log H. volcanii YL6 and YL7 grown with 0.2 mM Trp. mCherry (magenta) and GFP (green) fluorescence was quantified by plotting normalized intensity in the respective channel along the ring band. Scale bars, 1 µm. Source data are provided as a Source Data file. c 3D-SIM images (xy-tilted) of mid-log H. volcanii YL29 (H26 + CdpA-GFP), YL24 (H26 + FtsZ1-GFP), and YL26 (H26 + FtsZ2-GFP) grown with 0.2 mM Trp. Scale bars, 0.5 µm. The data shown in panel b and c are representative of at least two independent experiments.
