Fig. 4: Division protein localization dynamics and the effect of cdpA deletion on FtsZ1 and FtsZ2 movement.

Cells were grown in Hv-Cab without tryptophan induction to minimize the expression level of fluorescent fusion proteins. Mid-log cultures were sampled for TRIF imaging. The data shown is representative of at least two independent experiments. a Maximum intensity projections (MIPs) of two representative wild-type H26 cells expressing FtsZ1-GFP (i and ii) are shown on the left, with the corresponding kymographs at positions indicated by red arrows in the MIPs shown on the right. b MIPs of two representative wild-type H26 cells expressing FtsZ2-GFP (i and ii) and their corresponding kymographs. c, d MIPs of wild-type H26 cells expressing CdpA-GFP and their corresponding kymographs with different imaging time-window (4 s intervals for 15 min, c; 10 s intervals for 40 min, d). e MIP of one representative ΔcdpA expressing FtsZ1-GFP and its kymograph computed from the intensity along the long axis of cell (between the two red arrows). The relative position (0-1) along the long axis of cell is labelled below the kymograph. f Montages of FtsZ1-GFP dynamics in ΔcdpA and the integrated fluorescence time trace for the ROI region, as shown as a small red box around the centre of ring at the start of imaging (Time 0 min). The red curve is the moving average (every 5 points) of the raw intensity (grey dots). Source data are provided as a Source Data file. g The mean power spectral density (PSD) curves (± SEM) of FtsZ1-GFP in wild-type and ΔcdpA cells (n = 21). Source data are provided as a Source Data file. Montages of representative FtsZ2-GFP dynamics in two ΔcdpA cells and their corresponding kymographs indicate the changes in ring position (h) and orientation (i). All scale bars are 1 µm.