Fig. 5: The three domains of CdpA have different contributions to the role of CdpA in of the organisation of FtsZ1 and FtsZ2.
From: Cell division protein CdpA organises and anchors the midcell ring in haloarchaea
a Alphafold2 models of CdpA showing domain boundaries of the GFP tagged deletion constructs, including the N-terminal domain (NTD), disordered linker, and C-terminal domain (CTD). b Fluorescence microscopy of H26 wild-type strains expressing the indicated constructs during mid-log growth in Hv-Cab medium with 0.2 mM Trp. Arrows indicate regions of weak midcell localisation. Scale bars, 5 µm. The lower panels show demographic analyses of fluorescence along the long axis, ordered from shortest to longest cells (left to right). n=number of cells examined over one representative experiment. c Phase-contrast and fluorescence micrographs of the indicated cdpA deletion strains producing either FtsZ1-mCherry or FtsZ2-GFP, sampled during mid-log phase growth in Hv-Cab + 0.2 mM Trp. Arrows indicate some faint foci of FtsZ2-GFP. Scale bars, 5 µm. d Fluorescence images of the indicated cdpA deletion strains co-producing FtsZ1-mCherry and FtsZ2-GFP. Scale bars, 2 µm. e Distributions of cellular correlation coefficients between FtsZ1-mCherry and FtsZ2-GFP and (f) ratio of localisation area for FtsZ1-mCherry:FtsZ2-GFP in the background strains shown below the lower panel. The sample sizes analysed for each background strain in panel e were as follows: 587 for H26, 665 for ΔcdpA, 397 for cdpAΔNTD, 424 for cdpAΔLinker, and 386 for cdpAΔCTD. The statistical test in panel e was performed using an unpaired t-test (two-tailed), with **** indicating a significant difference (P < 0.0001). The sample sizes analyzed for each background strain in panel f were as follows: 471 for H26, 320 for ΔcdpA, 349 for cdpAΔNTD, 329 for cdpAΔLinker, and 447 for cdpAΔCTD. (112 data points fell outside the axis limits). The data shown are representative from one of two independent experiments. Source data are provided as a Source Data file.
