Fig. 1: TaHAKAI is a candidate gene for resistance to wheat yellow mosaic disease.
From: An m6A methyltransferase confers host resistance by degrading viral proteins through ubiquitination
A, G Assessment of tahakai, L1-T2 and L7-T2 in the WYMV disease nursery at Yangzhou, Jiangsu Province; JING 411 and YM158 were used as controls. Determination of viral RNA replication levels via qRT‒PCR. The value is the mean ± SD (two-sided t-test; three independent biological replicates were included, ‘**’ p < 0.01, p = ‘0.0033’ in (B), ‘0.0008’ in (E) and <0.0001 in H, respectively). C, F, I Determination of WYMV protein accumulation using western blot analysis. The value is the mean ± SD (three independent biological replicates were included). Ponceau staining in (E, H, K) shows the protein loaded in the assays. D Phenotypes in the fourth leaves of the plants inoculated with BSMV: 00, BSMV: PDS, BSMV: TaHAKAI and WYMV. J DNA polymorphisms in the TaHAKAI locus among 241 cultivated accessions. The TaHAKAI gene and SNPs are shown on the physical chromosome map. The blue boxes represent exons, the grey boxes represent introns, and the different coloured lines represent different SNP variant types. The different haplotypes of Haps were compared with those of Hap1. K Box plot of WYMV resistance comparisons between varieties harbouring the Hap1-3 haplotypes. Here, n represents the number of wheat accessions with the corresponding haplotype. Statistics: A two-sided t-test was performed, ‘*’ p < 0.05, ‘***’p < 0.001. Hap1 vs Hap2, p = ‘0.1363’; Hap1 vs Hap3, p < ‘0.0001’; Hap2 vs Hap3, p = ‘0.0053’. n = 209 (Hap1), 23(Hap2) and 9 (Hap3), respectively. boxplots indicate median (box centre line), 25th, 75th percentiles (box), and 5th and 95th percentiles (whiskers).
