Fig. 5: YoaA-χ binds substrates used in helicase assays when SSB is bound.
From: The YoaA-χ helicase modulates the dynamics of single-stranded DNA binding protein on DNA
A Relative intensity of fluorescein (glowing green circle) located either 4-, 7-, 11-, 16-, or 20-nt away from ds/ss junction on an overhang substrate (50 nM, O9) in the presence of either 1 μM YoaA-χ (blue) or DinG (orange). Relative intensity of fluorescein located 7-nt away from the ds/ss junction on an overhang substrate (50 nM, O10) is shown when either (B) YoaA-χ (50 nM, 100 nM, 250 nM, 500 nM, 1 μM, or 1.5 μM) or (C) DinG (50 nM, 100 nM, 250 nM, 500 nM, 1 μM, or 1.5 μM) is added. Relative intensity of fluorescein located 7-nt away from the ds/ss junction on a forked substrate (50 nM, F10) is shown when either (D) YoaA-χ (50 nM, 100 nM, 250 nM, 500 nM, 1 μM, or 1.5 μM) or (E) DinG (50 nM, 100 nM, 250 nM, 500 nM, 1 μM, or 1.5 μM) is added. For panels (B–E) black indicates naked DNA, green indicates SSB (75nM) prebound to DNA, and pink indicates SSB ΔC1 (75 nM) prebound to DNA. Dots represent individual experiments, dashed lines represent averages of these three experiments, and error bars indicate standard deviation. Source data are provided as a source data file.
