Fig. 7: YoaA-χ pulls SSB while it translocates along ssDNA.
From: The YoaA-χ helicase modulates the dynamics of single-stranded DNA binding protein on DNA
All kymographs were collected with biotinylated ssDNA (20,452-nt) tethered between two streptavidin beads at constant force of 10 pN. A Representative kymograph of ssDNA in a channel containing AF647-labeled SSB (red, 20 pM) and ATP (4 mM). B A kymograph is shown for the same DNA strand that was used in panel (A) and moved to a different channel containing AF647-SSB (red, 20 pM), YoaA-χ (100 nM), and ATP (4 mM). C Representative kymograph of ssDNA in a channel with AF647-labeled SSB (red, 20 pM), ATP (4 mM), and YoaA-χ R128A (100 nM). D Binding events of each experimental condition were binned into intervals of 2 s and shown in histograms. The SSB negative control (top, green) contained 442 total tracks measured on 22 different DNA molecules. Wild-type YoaA-χ (middle, gray) contained 745 total tracks measured on 15 different DNA molecules. YoaA-χ R128A (bottom, purple) contained 490 total tracks measured on 35 different DNA molecules. Source data are provided as a source data file.
