Fig. 7: Expression and structure of the HD genes in Microbotryum fungi.
From: Repeated loss of function at HD mating-type genes and of recombination in anther-smut fungi
A HD1 and HD2 gene and protein structures and functional domains (N-terminal to C-terminal), in two strains of M. superbum (1065 and 6P/6D), and one strain of each of M. shykoffianum, M. scorzonerae and M. lychnidis-dioicae. Protein sequence length is given in number of amino-acids (aa). Intron positions are shown with a black triangle and homeodomain positions by a dashed rectangle. Identical amino-acid sequences are shown with identical colors. B Expression analysis of HD genes. Log2 ratio of the HD1-to-HD2 TPM (transcripts per million of mapped reads) in mated conditions of two Microbotryum species (M. lychnidis-dioicae and M. superbum). Balanced expression generates Log2 ratios close to zero (dashed line). Data is shown for both alleles of M. lychnidis-dioicae. The diploid M. superbum strain used was homozygous at the HD gene. Normalized read counts were averaged for both genes predicted at the locus homologous to HD2 in M. superbum 6P. The different replicates are shown for each species (48-1 and 48-2 for M. lychnidis-dioicae and A, B and C for M. superbum). C Genome tracks showing, in each of M. lychnidis-dioicae a1 (top), M. lychnidis-dioicae a2 (middle) and M. superbum (bottom), the HD locus at the top and the per-position read mapping depth at the bottom, along genome position, in a representative RNAseq sample per species in mating conditions. Arcs represent splitted RNAseq reads showing perfect splicing of the inferred introns. Note that RNAseq reads were not stranded, so the strand-of-origin cannot be identified. RNAseq read mapping was displayed with jbrowse 2. Source data are provided as a Source Data file.
