Fig. 5: Functional characterization of AtMC9 and its mutants.
From: Structural determinants for pH-dependent activation of a plant metacaspase
The GRRase activity is shown as relative fluorescence units (RFUs) for 60 min with 1-min intervals for H193A (black). E112K (pink), H208A (green), WT (orange), H307A (blue), E255A (purple), and C147G (red) under indicated pHs for 60 min with 1-min intervals. Data are presented as mean ± standard deviation (SD). Error bars represent SD (n = 3 independent technical replicates). a 3.6; b 4.6; c 5.6; d 6.6; e 7.6; f 8.6; g 9.6. h The RFU range for AtMC9 and its mutants at 7 different pHs at the 20 min time point: +++++, >40,000; ++++, >20,000; +++, >10,000; ++, >5000; +, >1000; ±, >200; −, 0. Activities lower than WT are highlighted in blue, and those higher in salmon. Source data are provided as a Source Data file.
