Fig. 3: Mass spectrometry-based profiling of AEV proteomes.
From: Proteomic and metabolomic profiling of extracellular vesicles produced by human gut archaea
a Principal component analysis (PCA) plot illustrating the protein profiles of AEVs and WCLs of M. smithii ALI and M. intestini, including only proteins detected in all three replicates per group (AEV M. smithii ALI, AEV M. intestini, WCL M. smithii ALI, WCL M. intestini). b Overlap of 229 proteins identified in the AEVs of M. smithii ALI (left, n = 3 biological replicates) and M. intestini (right, n = 3 biological replicates), visualized and organized by intensities/relative abundance (circle size) and functional categorization (see Supplementary Data 6–8 for details). Data were visualized using RawGraphs113 and InkScape104. c Bar chart displaying mean intensities/relative abundances of protein categories in AEVs and WCLs, based on proteins detected in all three biological replicates of both M. smithii ALI and M. intestini (n = 229) (Supplementary Data 6–8). d Heatmap showing enrichment of 46 proteins annotated as adhesin/adhesion/IG-like present in all six AEV extracts (three biological replicates each of AEV M. smithii ALI and AEV M. intestini) compared to the whole cell lysates based on relative abundances. WCL whole cell lysate, AEV archaeal extracellular vesicles. Source data are provided as a Source Data file.
