Fig. 2: DNA content of M. smithii EVs.
From: Biogenesis of DNA-carrying extracellular vesicles by the dominant human gut methanogenic archaeon
A Coverage depth across the M. smithii PS chromosome. Each dot represents the coverage at the indicated position. B Coverage depth across distinct regions of the M. smithii PS chromosome: region i (nucleotide coordinates 76–80 kb) giving rise to a circular element of 2.9 kb; region ii (nucleotide coordinates 500–1000 kb), representing the typical sequencing depth across the genome; region iii (nucleotide coordinates 1692 – 1732 kb), encompassing the MSTV1 provirus. Source data for Fig. 2A, B are provided as a Source Data file. C Detection of the integrated and excised form of the 2.9 kb DNA fragment in both cell and purified EV preparations. The agarose gel electrophoresis shows the PCR amplified products: lane 1, integrated element within the host chromosome (expected size: 291 bp); lane 2, excised and circularized form of the 2.9 kb DNA element (expected size: 433 bp). D Genetic map of the circular element. The ORFs are represented by arrows indicating the direction of transcription. CofH, 5-amino-6-(D-ribitylamino)uracil:L-tyrosine 4-hydroxybenzyltransferase; ComD, sulfopyruvate decarboxylase subunit alpha. Genes msm_0079 and msm_0080 are preceded by divergent promoters, which are indicated with broken arrows.
