Fig. 3: Analysis of protein content of M. smithii EVs.
From: Biogenesis of DNA-carrying extracellular vesicles by the dominant human gut methanogenic archaeon
A Functional classification of proteins identified in purified M. smithii EVs using archaeal clusters of orthologous groups (arCOGs). Annotations for the arCOG categories are provided in Supplementary Data 2. B Correlation between protein abundances in M. smithii EVs and cells. Simple linear regression was performed, and the best fit line is shown (intercept = − 2.72, slope = 0.80, p-value < 2.2e-16, R2 = 0.6004). C Volcano plot comparing protein content of M. smithii EVs and cells. Read lines highlight the threshold for enrichment (p = 0.05 and |fold change | = 1.5). Differential protein abundances and adjusted p-values were calculated using the package DEP (Differential Enrichment analysis of Proteomics data). Blue dots indicate individual proteins significantly enriched in EVs. D Proteins significantly enriched in EVs when compared to proteins in the cellular fraction. The source data for Fig. 3 are provided as a Source Data file.
