Fig. 4: ATP and tension dependencies of DNA rewinding events.

a Bar plots shows the probability of rewinding events following unwinding for the WT helicase (blue bars, N = 46), ΔZBD (orange bars, N = 25), and ΔC-tail (magenta bars, N = 36) at varying ATP concentrations. b Average rewinding/unwinding ratios for the WT (blue symbols, N = 17), ΔZBD (orange symbol, N = 4) and ΔC-tail (magenta symbols, N = 29) as a function of ATP concentration. Ratios ~1 indicate that the number of rewound bp is similar to the number unwound bp. Data for the ΔZBD variant is shown only at 10 mM ATP due to insufficient rewinding events at lower ATP concentrations for meaningful analysis. c Average rewinding velocities for the WT (blue symbols, N = 20), ΔZBD (orange symbol, N = 4) and ΔC-tail (magenta symbols, N = 25) variants as a function of ATP concentration. d Probabilities of rewinding events following unwinding for the WT helicase (blue bars, N = 45), ΔZBD (orange bars, N = 32) and ΔC-tail (magenta bars, N = 27) variants at varying mechanical tension. e Average rewinding/unwinding ratios for the WT (blue symbols, N = 15), and ΔC-tail (magenta symbols, N = 24) variant as a function of tension. f Average rewinding velocities for the WT (blue symbols, N = 16) and ΔC-tail (magenta symbols, N = 23) variant under varying tension. ATP dependencies were measured at 6 pN and tension dependencies were measured at 4 mM ATP. Data points represent the average of multiple independent measurements, and error bars indicate the s.e. For this figure (b–f) source data are provided as a Source Data file.