Fig. 3: Structural basis of type 1 pilus assembly termination through incorporation of FimI. | Nature Communications

Fig. 3: Structural basis of type 1 pilus assembly termination through incorporation of FimI.

From: Structures of the Escherichia coli type 1 pilus during pilus rod assembly and after assembly termination

Fig. 3

a Composite cryo-EM map of the FimDHGFAnICHis complex (map XV (pilus rod) and map XVI (assembly platform)). b Cartoon representation of the FimDHGFAnICHis complex (composite of model XV and model XVI). The complex is colored according to the color scheme displayed in a. c Structural alignment of the FimA-bound (FimDHGFAnC complex, model IV) and FimI-bound usher (FimDHGFAnICHis complex, model XVI) based on the transmembrane and plug domain of FimD (residues 140–665; RMSD = 0.56 Å). The FimA-bound usher is colored gray while the FimI-bound usher is colored according to the same color scheme as in a. d Detail of the alignment in (c) displaying the interaction between the FimA subunit occupying the pore and the chaperone FimC in the FimA-bound (gray) and FimI-bound (colored) usher complexes. The patterns of potential inter-subunit hydrogen bonds are nearly identical in both structures.

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